Purification and Characterization of Recombinant Anthrax Edema Factor
نویسندگان
چکیده
منابع مشابه
Purification of anthrax edema factor from Escherichia coli and identification of residues required for binding to anthrax protective antigen.
The structural gene for anthrax edema factor (EF) was expressed in Escherichia coli under the control of a powerful T5 promoter to yield the 89-kDa recombinant protein that reacted with anti-EF antibodies. Recombinant EF was purified to homogeneity by a two-step procedure involving metal chelate affinity chromatography and cation-exchange chromatography. From 1 liter of culture, 2.5 mg of biolo...
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Bacillus anthracis is the causative agent of anthrax, and the tripartite anthrax toxin is an essential element of its pathogenesis. Edema factor (EF), a potent adenylyl cyclase, is one of the toxin components. In this work, anti-EF monoclonal antibodies (MAb) were produced following immunization of mice, and four of the antibodies were fully characterized. MAb 3F2 has an affinity of 388 pM, was...
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ژورنال
عنوان ژورنال: Journal of the Korea Institute of Military Science and Technology
سال: 2011
ISSN: 1598-9127
DOI: 10.9766/kimst.2011.14.4.710